Endotoxin assay by bioluminescence using mutant firefly luciferase
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چکیده
منابع مشابه
Endotoxin assay by bioluminescence using mutant firefly luciferase.
The Limulus reaction is an application of the defense mechanism of horseshoe crab for endotoxin detection. Endotoxin is a component of the cell wall in the outer membrane of gram-negative bacteria, and causes fever or shock when it enters the human blood stream. For endotoxin detection, gel formation or turbidity of the coagulation factor chromogen or fluorescence-modified peptide is used. Howe...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2010
ISSN: 0003-2697
DOI: 10.1016/j.ab.2009.10.025